N-2-гидроксиэтилпиперазин-N'-2-этансульфоновая кислота (HEPES, Free Acid), Buffer grade, AppliChem, 250 г
8 263 руб.
- Производитель: AppliChem
- Каталожный номер:
A1069,0250 - CAS номер : 7365-45-9
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Характеристики реактива
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HEPES for buffer solutions A1069
Synonym |
4-(2-Hydroxyethyl)-1-piperazine-1-ethanesulfonic Acid, |
useful pH range pK a (20°C) Melting point |
pH 6.8 - 8.2 7.55 210 - 215°C |
Formula M CAS-No.: HS-No.: EC-No.: |
C 8 H 17 N 2 NaO 4 S 238.31 g/mol 7365-45-9 29335995 230-907-9 |
Storage: LGK: Disposal: |
RT 10 - 13 3 |
WGK: |
1 |
Specification Assay (titr.) pH (1 %; H 2 O) Heavy metals (as Pb) Water Chloride Sulfate |
min. 99.5 % 4.7 - 6.0 max. 0.001 % max. 0.05 % max. 0.05 % max. 0.05 % |
Literature |
Comment
Hepes is a widely used buffer in biological studies. There is only one restriction in the use of this buffer,
because it interferes with the Folin protein assay. In cell culture media, it is employed as a substitute for the
bicarbonate buffer at a concentration of 25 mM or as a supplement to the bicarbonate buffer (concentration 10 - 15
mM).
The addition of 20 mM HEPES to TBE buffer improves the migration behaviour of certain samples in the SSCP
analysis (single-strand conformation polymorphism), with a higher resolution especially if small differences in the
sequences between certain bands are wanted (5).
Характеристики реактива | |
Фасовка | 250 г |