AБТС(ABTS) 2,2'-азино-бис-(3-этилбензтиозолин-6-сульфокислоты) диаммониевая соль, для биохимии, Applichem, 5 г
33 619 руб.
- Производитель: AppliChem
- Каталожный номер:
A1088,0005 - CAS номер : 30931-67-0
ABTS® BioChemica
Synonym |
2,2'-Azino-bis-(3-Ethylbenzthioazoline-6-Sulfonic Acid) |
state of matter |
Solid |
Melting point |
> 300°C (dec.) |
Formula M CAS-No.: HS-No.: EC-No.: |
C 18 H 24 N 6 O 6 S 4 548.69 g/mol 30931-67-0 29339980 250-396-6 |
Storage: LGK: |
2-8°C 10 - 13 |
R: S:
WGK: |
36/37/38 26-36 irritant 3 |
Specification TLC E 0.001 %/1 cm,340 nm Solubility (2 %; H 2 O) Loss on drying |
passes test min. 38000 clear, green max. 6 %
|
Literature (1) Childs, R.E. & Bardsley, W.G. (1975) Biochem. J. 145, 93-103 The steady-state kinetics of peroxidase |
Comment
At concentrations above 56 mM, ABTS is insoluble. Heating facilitates dissolution of ABTS, e. g. in 0.1 M
phosphate buffer. If it crystallizes upon cooling (e. g. at concentrations of 1 mg/ml), the supernatant can be used.
This behavior can be used to separate excess of ABTS (2). At concentrations below 0.002 mM, it may happen, that,
depending on the peroxidase concentration, the substrate ABTS will be completely used up and measurements of
enzyme activity will be carried out in the non-linear range. Absorption maxima of ABTS are found at 340 nm and
414 nm, respectively. Usually, measurements will be performed at 420 nm.
Solutions of ABTS in 0.5 M phosphate-citrate buffer (pH 4.0) are stable for at least one month, if stored at +4°C
(5).
A1088
Характеристики реактива | |
Фасовка | 5 г |