Yeast Nitrogen Base With Amino Acids Non-selective yeast growth medium Sigma Y1250
Components
Component, amount (μg/L unless indicated)
Nitrogen Sources:
Ammonium sulfate, 5.0 g/L
Amino Acids:
L-histidine, 10 mg/L
DL-methionine, 20 mg/L
DL-tryptophan, 20 mg/L
Vitamins:
Biotin, 2.0
Calcium pantothenate, 400
Folic acid, 2.0
Inositol, 2.0 mg/L
Nicotinic acid, 400
p-Aminobenzoic acid, 200
Pyridoxine HCl, 400
Riboflavin, 200
Thiamine HCL, 400
Trace Elements:
Boric acid
Copper sulfate
Potassium iodide
Ferric chloride
Magnesium sulfate
Sodium molybdate
Zinc sulfate
Salts:
Potassium phosphate monobasic, 1.0 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L
General description
Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains amino acids, nitrogen, vitamins, trace elements and salts.
Packaging
250 g in poly bottle
Reconstitution
Make a 10x concentrated stock solution by stirring to suspend 6.7g yeast nitrogen base powder and 5 g glucose in 100 mL water. Warm if necessary to aid solubilization. Filter sterilize and store at 2-8°C.
Dilute the 10x concentrated stock to a 1x working solution by adding 100mL concentrated stock to 900mL sterile water.
Характеристики реактива | |
grade | for molecular biology |
storage temp. | room temp |