X-GlcA reagent for selection of recombinant bacterial clones Sigma B6650
По запросу
- Производитель: Sigma
- Каталожный номер:
B6650 - CAS номер : 114162-64-0
Application
Chromogenic substrate for β-glucuronidase (GUS) gene detection.
Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.
General description
X-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Principle
When X-GlcA is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of X-GlcA turn an intense blue color, allowing for easy differentiation between lac+ and lac- colonies.
Характеристики реактива | |
assay | ≥98% (TLC) |
grade | Molecular Biology Reagent, for molecular biology |
Molecular Formula | C14H13BrClNO7 · C6H13N |
storage temp. | −20°C |