RNase B Glycoprotein Standard from bovine pancreas Proteomics Grade Sigma R1153
По запросу
- Производитель: Sigma
- Каталожный номер:
R1153 - CAS номер : 9001-99-4
Frequently Asked Questions
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Application
RNase B is a preferred substrate with PNGase F for demonstration of N-linked deglycosylation using SDS-PAGE or MALDI-MS. The activity of PNGase F is routinely assayed using RNase B by monitoring the pronounced mobility shift in 12% gels after deglycosylation. Proteomics Grade RNase B can also be used as a source of N-glycans following enzymatic digestions and subsequent purification.
Other Notes
Bovine pancreatic RNase B is a glycoprotein that contains only N-linked glycans. It is a globular protein composed of a single domain that occurs naturally as a lesser component in mixture along with ribonuclease A (RNase A), which is the non-glycosylated core form. RNase B contains a single glycosylation site at Asn34 at which from five to nine mannose residues are attached to the chitobiose core, i.e. Man5-9GlcNAc2. Due to the heterogeneity in the glycosylation at Asn34, RNase B exists as five glycosylated varients, with a molecular weight of approx. 15 kDa.
Quality
RNAse B Glycoprotein Standard has been highly purified to remove contaminating RNase A.
Характеристики реактива | |
assay | ≥90% (SDS-PAGE) |
grade | Proteomics Grade |
storage temp. | −20°C |