Z34 Reactivity: Anti-IgG2a and IgG2b (allotype dependent), mouse Sigma 93022521
По запросу
- Производитель: Sigma
- Каталожный номер:
93022521 - CAS номер :
Immunogen
A/PR/8/34 mouse pathogenic human influenza virus
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Application
Differential detection of complexed IgG2a in the presence of native IgG2a, allotype detection or quantitation.
Cell Line Origin
Mouse Sp2/0 x Mouse BALB/c spleen
Culture Medium
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS) (can be adapted to 1% Foetal Bovine Serum (FBS))
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Cell Line Description
The immunisation protocol involved repeated intranasal infection and a single i.v. injection before fusion. The antibody binds murine IgG2a of "a" and "b" allotype, Fc region. The isoallotype-specific Z34 IgA shows cross-reactivity with IgG2b ("b" allotype) of C57BL/6 origin but not of BALB/c origin. There is no cross-reaction with A/PR/8/34 influenza virus, DNP, NP, DNA or cytoskeletal proteins. Contains a CH2 domain.
Характеристики реактива | |
storage temp. | −196°C |