YTA 3.1.2 Reactivity Anti-L3T4 epitope B, mouse CD4 ) Sigma 89040603
По запросу
- Производитель: Sigma
- Каталожный номер:
89040603 - CAS номер :
Application
Not specified
Immunogen
CBA mouse spleen cells, thymocytes
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Cambridge Enterprise Limited, Hauser Forum, 3 Charles Babbage Road, Cambridge CB3 0GT. Originators: Professor H Waldmann and Dr SP Cobbold when at the Department of Pathology, University of Cambridge
Cell Line Origin
Not specified
Culture Medium
Iscoves Modified Dulbeccos Medium (IMDM) + 2 mM glutamine + 5% Fetal Bovine Serum (FBS). Where the cells are grown under static conditions, 0.02% EDTA, trypsin or mechanical agitation maybe required to dislodge them from the plastic.
Subculture Routine
Maintain cultures between 3-9 x 105 cells/ml; 5% CO2; 37 °C. Saturation density 9 x 105/ml
Cell Line Description
The antibody is directed against epitope B of the L3T4 antigen. This antigen is the murine equivalent of CD4. Has been used in CD4 subset depletion in vivo and flow cytometry studies. Cambridge Enterprise Reference number AB13:www.enterprise.cam.a
Характеристики реактива | |
storage temp. | −196°C |