YO Sigma 85110501
По запросу
- Производитель: Sigma
- Каталожный номер:
85110501 - CAS номер :
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Dr J Jarvis, MRC Laboratory of Molecular Biology, Cambridge
Application
Hybridisation and cloning studies
Cell Line Origin
Rat myeloma
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
Cell Line Description
The full designation of this line is YB2/3.0Ag30. YO was derived from a hybrid myeloma YB2/3HL by cloning in soft agar to select a non-secreting sub-population. The cells are azaguanine resistant and do not secrete IgG.
Характеристики реактива | |
storage temp. | −196°C |