XrS6-hamKu80 Sigma 96012301
По запросу
- Производитель: Sigma
- Каталожный номер:
96012301 - CAS номер :
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Dr P Jeggo, MRC Cell Mutation Unit, University of Sussex
Application
Study of DNA repair mechanisms
Cell Line Origin
Hamster Chinese ovary, transformed with hamster Ku80 cDNA
Culture Medium
Ham′s F12 + 2mM Glutamine + 300μg/ml G418 + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Cell Line Description
The cell line Xrs6-hamKu80 was derived from a radiosensitive CHO-K1 mutant transfected with hamster Ku80 cDNA. Ku80, a subunit of the Ku protein and component of the DNA-dependent protein kinase, is a gene product defective in radiosensitive CHO cells, so called xrs mutants. XRC5, the gene encoding Ku80, belongs to the ionisation radiation group affected in DNA repair defects. Mutants transfected with hamster Ku80 obtained full restoration of gamma-ray resistance, DNA end-binding activity to parent levels and expression of Ku80 and Ku70 proteins.
Характеристики реактива | |
storage temp. | −196°C |