XC Sigma 88120601
По запросу
- Производитель: Sigma
- Каталожный номер:
88120601 - CAS номер :
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Application
Plaque assay and virus studies: Rous sarcoma virus, MuLV
Cell Line Origin
Rat Wistar sarcoma
Culture Medium
EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
Cell Line Description
Original tumour induced in outbred Wistar rats by intramuscular injection of Rous sarcoma virus. The cells have virus genome but do not normally release infectious virus. The XC plaque assay is an indirect method of detection for MuLV in cultured cells.
Характеристики реактива | |
storage temp. | −196°C |