WRL 68 (HeLa derivative) Sigma 89121403
По запросу
- Производитель: Sigma
- Каталожный номер:
89121403 - CAS номер :
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Application
In vitro hepatic model
Cell Line Origin
Human Negroid cervix carcinoma
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 12,14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 1-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
Cell Line Description
The human hepatic cell line WRL-68 exhibits a morphology similar to hepatocytes and hepatic primary cultures. Cells have been shown to secrete albumin and α-feto protein and express liver specific enzymes such as alanine amino transferase, aspartate amino transferase, gamma-glutamyl transpeptidase and alkaline phosphatase. Previously contaminated with Mycoplasma; treated and cured at ECACC. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.
Характеристики реактива | |
storage temp. | −196°C |