WRC Sigma 88031148
По запросу
- Производитель: Sigma
- Каталожный номер:
88031148 - CAS номер :
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Dr E I Levene, Department of Pathology, University of Cambridge
Application
Tumourigenicity and virus studies: HSV, PRV and vaccinia viruses
Cell Line Origin
Rat Walker carcinoma
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Cell Line Description
Derived from the parent line LLC-WRC 256, which was established in 1954. The cells are tumorigenic in suckling rats and support the growth of HSV, PRV and Vaccinia viruses.
Характеристики реактива | |
storage temp. | −196°C |