WM-115 Sigma 91061232
По запросу
- Производитель: Sigma
- Каталожный номер:
91061232 - CAS номер :
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Application
Tumourigenicity studies
Cell Line Origin
Human melanoma
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12,13
D16S539: 11,12
D5S818: 13
D7S820: 8
THO1: 7,9
TPOX: 8,11
vWA: 15,17
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Cell Line Description
Derived from a primary epitheloid tumour. Expresses proteoglycan antigen characteristic of melanomas. Tumourigenic in nude mice. See also WM 266-4 (Sigma Catalogue number 91061233) which is derived from a metastasis from the same patient.
Характеристики реактива | |
storage temp. | −196°C |