WIL2.NS.6TG Sigma 93031001
По запросу
- Производитель: Sigma
- Каталожный номер:
93031001 - CAS номер :
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Application
Immortalising antigen-stimulated human B-lymphocytes by somatic hybridisation
Cell Line Origin
Human Caucasian B lymphocyte
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 5x1,000-5x10,000 cells/ml; 5% CO2; 37°C.
Cell Line Description
A non Ig-secreting, HAT sensitive, 6-thioguanine resistant, human B lymphocyte. Derived from the parent line WIL2.NS (Sigma Catalogue number. 90112121).
Характеристики реактива | |
storage temp. | −196°C |