W3/25 Reactivity: Anti-CD4, rat Sigma 84112002
По запросу
- Производитель: Sigma
- Каталожный номер:
84112002 - CAS номер :
Immunogen
Rat thymocyte membrane
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Cell Line Origin
Mouse NS1/1 x Mouse BALB/c spleen
Culture Medium
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO2; 37°C.
Cell Line Description
The antibody is directed against surface antigens found on 86% of rat thymocytes, 70% of peripheral T lymphocytes and macrophages. The antigen has a molecular weight of approximately 50kDa. W3/25 positive T cells have been identified as T helper cells.
Характеристики реактива | |
storage temp. | −196°C |