VP229 Sigma 05092804
По запросу
- Производитель: Sigma
- Каталожный номер:
05092804 - CAS номер :
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Cell Line Origin
Human Breast Cancer
Culture Medium
MEBM (Clonetics, CC-3151) + MEGM SingleQuots (Clonetics, CC-4136) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS). Fresh EGF (final concentration of 10ng/ml) should be added to the medium at each subculture.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. The VP series of breast cancer cell lines are very slow growing and relatively difficult to grow. Fresh EGF (final concentration 10ng/ml) s
Cell Line Description
Derived from a breast cancer biopsy removed from a 47 year-old female. The histology of the breast cancer biopsy was defined as Ductal Grade III, tumour cells did not stain positive for oestrogen receptors. The VP229 cell line was derived from the same individual as the cell line VP267. VP229 was derived from the primary tumour and VP267 was derived 14 months later from a local recurrence of the tumour. The individual was recorded to live for 2 years after surgery performed to remove the primary tumour. This cell line grows slowly and is relatively difficult to culture; please read the sub-culture routine information closely.
Характеристики реактива | |
storage temp. | −196°C |