Polyethylene glycol solution average mol wt 1,450, Hybri-Max™, 50 % (w/v), sterile-filtered, BioReagent, suitable for hybridoma Sigma P7306
По запросу
- Производитель: Sigma
- Каталожный номер:
P7306 - CAS номер : 25322-68-3
Краткое описание
PEG solution... Читать далее →
Характеристики реактива
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Application
PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.
Recommended for use in a normal fusion protocol requiring 50% PEG and 10% DMSO.
Other Notes
Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) and 10% DMSO (v/v) in DPBS without Calcium.
Packaging
Packaged in sealed ampules under nitrogen.
Reconstitution
Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.
Legal Information
Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
Характеристики реактива | |
grade | Hybri-Max™ |
storage temp. | 2-8°C |