Gelatin from porcine skin gel strength 300, Type A Sigma G2500
По запросу
- Производитель: Sigma
- Каталожный номер:
G2500 - CAS номер : 9000-70-8
Components
Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.
Caution
Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.
Packaging
1 kg in poly bottle
100, 500 g in poly bottle
Preparation Note
This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gell strength of 300 and is soluble in water at 50 mg/mL.
Application
This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.
Gelatin was used for coating cell culture to improve attachment of cells and was added to PCR to help stabilize Taq DNA.1 It was used as a blocking reagent in Western blotting, ELISA, and immunohistochemistry.2 Gelatin can also be used as a component of media for species differentiation in bacteriology.3 Gelatin is a biocompatible polymer and has been used as delivery vehicle for the release of bioactive4 molecules and in the generation of scaffolds for engineering applications.5 Gelatin was used in the murine lethality assay, which is used to examine the ability of SMAbs to neutralize the effects of BoNT/A1 in vivo.6