β-Amanitin–poly-L -lysine bound ≥85% Sigma A8100
Caution
Highly toxic
Preparation Note
Supplied as 0.1 mg β-Amanitin bound to 0.5 mg poly-
Application
β-Amanitin inhibits RNA polymerase II and III in eukaryotic cells. β-Amanitin binds to the bridging helix of RNA polymerase II inhibiting the translocation of RNA and DNA required for transcription. β-Amanitin slows the rate of transcription roughly 1000 fold.
Biochem/physiol Actions
Toxic constituent of the mushroom, Amanita phalloides, inhibits eukaryotic RNA polymerase II and III, but not RNA polymerase I or bacterial RNA polymerase. Inhibits mammalian protein synthesis.
Характеристики реактива | |
assay | ≥85% |
storage temp. | 2-8°C |