Xanthine Oxidase from bovine milk Grade I, ammonium sulfate suspension, ≥0.4 units/mg protein Sigma X1875
По запросу
- Производитель: Sigma
- Каталожный номер:
X1875 - CAS номер : 9002-17-9
Analysis Note
Protein determined by biuret
General description
Formerly E.C. 1.1.3.22
Unit Definition
One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.
Physical form
Suspension in 2.3 M (NH4)2SO4 containing 1 mM sodium salicylate
Biochem/physiol Actions
Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids.1,2 It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines.3 It is also known to be a biological source of reactive oxygen species.4
Xanthine oxidase was shown to be involved in the reduction of cytochrome c by the generation of superoxide anions following the oxidation of xanthine. These free radicals are responsible for reducing cytochrome c.
Характеристики реактива | |
storage temp. | 2-8°C |