Trizma® hydrochloride BioUltra, for molecular biology, ≥99.0% (AT) Sigma 93363
По запросу
- Производитель: Sigma
- Каталожный номер:
93363 - CAS номер : 1185-53-1
Other Notes
Component of extraction buffer e.g. phenol extraction of DNA or RNA1; Buffer component of separating and stacking gels in the characterization of in vitro translation products by SDS-PAGE2.
Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.
Application
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma hydrochloride is used as a general reagent for the preparation of all types of Tris buffers.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
Характеристики реактива | |
assay | ≥99.0% (AT) |
grade | for molecular biology |
Molecular Formula | NH2C(CH2OH)3 · HCl |
pH | 2.5-4.0 (25 °C, 4 M in H2O) |