Trizma® base BioUltra, for molecular biology, ≥99.8% (T) Sigma 93362
По запросу
- Производитель: Sigma
- Каталожный номер:
93362 - CAS номер : 77-86-1
Краткое описание
2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol... Читать далее →
Характеристики реактива
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Other Notes
Running buffer component in non-denaturing agarose gel electrophoresis1; In non-denaturing and urea-denaturing polyacrylamide gel electrophoresis2; DNA digest analysis with capillary electrophoresis3.
Easily compare specifications for Trizma products with the Trizma specification table.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Application
Trizma is used in the formulation of buffer solutions in the pH range between 7.5 and 8.5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification. Trizma based buffers are also in column chromatography and in gel electrophoresis. Trizma base is used as a general reagent for the preparation of all types of Tris buffers.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
Характеристики реактива | |
assay | ≥99.8% (T) |
Boiling Point (°C) | 219-220 °C/10 mmHg(lit.) |
grade | for molecular biology |
Molecular Formula | NH2C(CH2OH)3 |
pH | 10.5-12.0 (25 °C, 4 M in H2O) |