Ribonuclease B from bovine pancreas BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE) Sigma R7884
По запросу
- Производитель: Sigma
- Каталожный номер:
R7884 - CAS номер : 9001-99-4
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Packaging
100 mg in poly bottle
500 μg in poly bottle
5 mg in glass bottle
Package size based on protein content
Preparation Note
Derived from New Zealand-sourced pancreas
Purified by affinity chromatography
Application
Ribonuclease B from bovine pancreas is used in the digestion of RNA during cell cycle platform analysis.1
Biochem/physiol Actions
Native RNase BS generated by subtilisin digestion of native RNase B comprising of amino acid residues 21-124 of RNase B, is sensitive to PNGase F digestion.2 Intramolecular N-glycans of bovine pancreatic RNase B function like chaperone. RNase B is found to be much faster than RNase A, while RNase A is liable to aggregate during regeneration. The stimulatory effect of Asn-oligosaccharide (which corresponds to the most predominant sugar chain of RNase B) reveals that the N-glycans of RNase B facilitates the transformation of bulky intermediates into folded, compact species.3
Характеристики реактива | |
assay | ≥80% (SDS-PAGE) |
storage temp. | −20°C |