Ribonuclease A from bovine pancreas Type I-AS, 50-100 Kunitz units/mg protein Sigma R5503
По запросу
- Производитель: Sigma
- Каталожный номер:
R5503 - CAS номер : 9001-99-4
Analysis Note
Protein determined by E
Features and Benefits
• RNase protection assays
• Remove unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolyze RNA contained in protein samples
• Purification of DNA
General description
Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can hydrolyze RNA from protein samples. Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds.
Packaging
1, 5, 10 g in poly bottle
100, 500 mg in poly bottle
Preparation Note
Derived from New Zealand-sourced pancreas
Salt fractionated and chromatographically purified.
Application
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess the tissue-specific expression of ruminant pancreatic type ribonucleases. Ribonuclease A from bovine pancreas has also been used in a study to investigate pancreas-specific glycosylated protein disulphide-isomerase binding to misfolded proteins and peptides.
Характеристики реактива | |
storage temp. | −20°C |