Ribonuclease A from bovine pancreas Type II-A, ≥60% (SDS-PAGE), 50-150 Kunitz units/mg solid Sigma R5000
По запросу
- Производитель: Sigma
- Каталожный номер:
R5000 - CAS номер : 9001-99-4
Analysis Note
Protein determined by E
RNase A purity determined by SDS-PAGE
Features and Benefits
• RNase protection assays
• Remove unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolyze RNA contained in protein samples
• Purification of DNA
General description
Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can hydrolyze RNA from protein samples. Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds.
Packaging
1 g in glass bottle
100, 250 mg in glass bottle
Preparation Note
Chromatographically purified
Application
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess hybridase activity of human ribonuclease-1. Ribonuclease A from bovine pancreas has also been used in a study to investigate particle-based and monolithic columns for cation exchange protein displacement chromatography.
Характеристики реактива | |
assay | ≥60% (SDS-PAGE) |
storage temp. | −20°C |