Ribonuclease A from bovine pancreas (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0) Sigma R4642
По запросу
- Производитель: Sigma
- Каталожный номер:
R4642 - CAS номер : 9001-99-4
Components
RNase A is supplied as a solution of 50% glycerol containing 10 mM Tris-HCl (pH 8.0).
Analysis Note
Protein determined by E
Application
Suitable for:
• RNase protection assays
• Removal of unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolysis of RNA contained in protein samples
• Plasmid DNA purification
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.
Features and Benefits
• RNase protection assays
• Remove unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolyze RNA contained in protein samples
• Purification of DNA
General description
RNase A is an endoribonuclease that attacks at the 3’OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA.
Other Notes
Activators of RNase A include potassium and sodium salts. RNase A can be inhibited by alkylation of His12 or His119.
Unit Definition
A major application for RNase A is the removal of RNA from preparations of plasmid DNA. For this application, DNase free RNase A is used at a final concentration of 10 ug/mL.
Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.
Характеристики реактива | |
grade | for molecular biology |
storage temp. | −20°C |