Ribonuclease A from bovine pancreas Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein Sigma R4875
По запросу
- Производитель: Sigma
- Каталожный номер:
R4875 - CAS номер : 9001-99-4
Analysis Note
Protein determined by E
Features and Benefits
• RNase protection assays
• Remove unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolyze RNA contained in protein samples
• Purification of DNA
Packaging
1, 5 g in glass bottle
100, 500 mg in glass bottle
Preparation Note
Salt fractionated and chromatographically purified.
Application
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays. RNase has been used as a tool for computer-aided drug design .
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.
Biochem/physiol Actions
Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3’ phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts.
Характеристики реактива | |
assay | ≥60% RNase A basis (SDS-PAGE) |
storage temp. | −20°C |