Phosphatase substrate 40 mg tablets Sigma P5994
По запросу
- Производитель: Sigma
- Каталожный номер:
P5994 - CAS номер : 333338-18-4
Application
Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
Phosphatase is a chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. It is been used for the colorimetric detection of CTB fusion proteins1. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.
The substrate has been used to determine the alkaline phosphatase (ALP) activity in human osteoblast-like Saos-2 cell lysates. ALP acts as an early marker of osteoblast differentiation that is expressed just before matrix mineralization begins.
Other Notes
Substrate for the determination of acid and alkaline phosphatases.
Packaging
25 tablets in glass bottle
Preparation Note
Dissolve tablets to the desired concentration in either of the following buffers: 0.1 M glycine, pH 10.4, with 1 mM MgCl2 and 1 mM ZnCl2 or 1 M diethanolamine, pH 9.8, with 0.5 mM MgCl2. Typically, a pNPP concentration of 1 mg/mL is used.
Quantity
Contains 40 mg substrate per tablet
Характеристики реактива | |
Molecular Formula | O2NC6H4OP(O)(ONa)2 · 6H2O |
storage temp. | −20°C |