Pepsin from porcine gastric mucosa Marker for denaturing PAGE Sigma P1143
По запросу
Наличие: Срок поставки 60 дней
- Производитель: Sigma
- Каталожный номер:
P1143 - CAS номер : 9001-75-6
Analysis Note
Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.
Application
Pepsin cleavage can be used to produce F(ab′)2 fragments of antibodies. pepsin at www.sigma-aldrich.com/enz
Biochem/physiol Actions
Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin
Характеристики реактива | |
storage temp. | 2-8°C |