Pepsin from porcine gastric mucosa Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, 3200-4500 units/mg protein Sigma SRE0001
По запросу
- Производитель: Sigma
- Каталожный номер:
SRE0001 - CAS номер : 9001-75-6
Analysis Note
Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5.
Protein determined by E
Application
Pepsin cleavage can be used to produce F(ab′)2 fragments of antibodies. pepsin at www.sigma-aldrich.com/enz
Biochem/physiol Actions
Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin
Other Notes
View more information on pepsin at www.sigma-aldrich.com/enz
Packaging
5, 10, 100, 250 g in glass bottle
Unit Definition
One unit will produce a ΔA280 of 0.001 per min at pH2.0 at 37° C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16mL. Light path = 1cm.)
Характеристики реактива | |
storage temp. | −20°C |