Papain from papaya latex lyophilized powder, aseptically filled Sigma P5306
По запросу
- Производитель: Sigma
- Каталожный номер:
P5306 - CAS номер : 9001-73-4
Application
Used to produce Fab fragments of antibodies.1 Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.3,4
Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies 1. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P5306 is provided as a lyophilized powder that is aseptically filled. It has been used to digest mouse retinas to study the RAGE axis in nonproliferative diabetic retinopathy (NPDR)2.
The product from Sigma has been used in the digestion of extracellular matrix (ECM).2 It has also been used with other proteolytic enzymes for in situ protein degradation to produce crystals suitable for structure determination.
Biochem/physiol Actions
A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.
Papain papaya latex has antifungal activity against C. albicans. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
Other Notes
View more information on papain at www.sigma-aldrich.com/enz
Preparation Note
Prepared from P 4762
Характеристики реактива | |
storage temp. | −20°C |