Neomycin trisulfate salt hydrate powder Sigma N1876
По запросу
- Производитель: Sigma
- Каталожный номер:
N1876 - CAS номер : 1405-10-3
Biochem/physiol Actions
Mode of action: This product acts by binding to the 30S and 50S subunits, causing miscoding and inhibiting initiation and elongation during protein synthesis. Neomycin also blocks voltage-sensitive Ca2+ channels without affecting the Na+/Ca2+ antiporter in neurons.
Antimicrobial spectrum: Neomycin acts against both gram-positive and gram-negative bacteria.
Caution
Stock solutions should be filter sterilized and stored at 2-8°C, and are stable at 37°C for 5 days. A 1 mg/mL neomycin solution in .1 M phosphate buffer, pH 8.0 should be used within 14 days when stored at 0-5°C. Solutions should be protected from light or moisture.
Packaging
25, 100 g in glass bottle
Preparation Note
Neomycin sulfate is soluble in H2O at 50 mg/mL, yielding a clear solution.
Application
Neomycin Trisulfate is produced by Streptomyces containing a minimum of 85% neomycin B. It has been used as a selection agent for prokaryotic cells that have been transformed using the selectable marker gene (neo), and to study ototoxic side effects of antibiotics, platelet-derived growth factor responses in certain fibroblasts, and extraction of Nuclear Phosphatidylinositol 4,5-Bisphosphate-Interact
Protocols & Applications
Характеристики реактива | |
Molecular Formula | C23H46N6O13 · 3H2SO4 · xH2O |