Kanamycin sulfate Streptomyces kanamyceticus powder, γ-irradiated Sigma K0879
По запросу
- Производитель: Sigma
- Каталожный номер:
K0879 - CAS номер : 25389-94-0
Application
Suitable for use as a selective agent for bacteria harboring a plasmid conferring kanamycin resistance (recommended use concentration 10-50 μg/mL). Also suitable for prevention of contamination in cell cultures (recommended use concentration 100 μg/mL).
Biochem/physiol Actions
Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding.
Mode of Resistance:Aminoglycoside
Antimicrobial spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.
General description
Kanamycin sulfate is an antimicrobial agent effective against Gram-negative and Gram-positive bacteria. It is used to prevent contamination in cell culture as well as a selective agent for bacteria with kanamycin resistance.
Principle
Kanamycin binds to the 30S ribosomal subunit and inhibits protein synthesis, leading to cell death.
Reconstitution
Prepare stock solution directly in the vial with sterile water to a final concentration of 10mg/mL. Stock solution should be stored at 2-8°C.
Protocols & Applications
Характеристики реактива | |
grade | for molecular biology |
Molecular Formula | C18H36N4O11 · H2O4S |
storage temp. | 2-8°C |