Isopropyl β-D -thiogalactopyranoside solution ReadyMade IPTG solution for Blue-white screening Sigma I1284
По запросу
- Производитель: Sigma
- Каталожный номер:
I1284 - CAS номер : 367-93-1
Components
IPTG Solution is composed of 200 mM IPTG in molecular biology grade water.
Application
Suitable for induction of recombinant protein expression in systems controlled by the lac repressor.
General description
Isopropyl thiogalactose (IPTG) is a sugar derivative widely used for the induction of the expression of recombinant proteins in E. coli. This expression system requires that the gene of interest is cloned in plasmids containing the Lac promoter.
Principle
Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for ß--galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product.
Характеристики реактива | |
grade | for molecular biology |
Molecular Formula | C9H18O5S |
storage temp. | −20°C |