Guanidine hydrochloride ≥99% (titration), organic base and chaeotropic agent Sigma G4505
По запросу
- Производитель: Sigma
- Каталожный номер:
G4505 - CAS номер : 50-01-1
Краткое описание
Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride... Читать далее →
Характеристики реактива
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Application
Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.
Guanidine hydrochloride is used in RNA isolation to dissociate nucleoproteins and inhibit RNase.
Caution
May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical. For free-flowing material, please see our Redi-DriTM free-flowing product number RDD001.
Packaging
1 kg in poly bottle
2, 10, 25 kg in poly drum
25, 100, 500 g in poly bottle
Preparation Note
In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes.
The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M.
Характеристики реактива | |
assay | ≥99% (titration) |
Molecular Formula | NH2C(=NH)NH2 · HCl |