Guanidine hydrochloride BioUltra, ≥99.5% (AT) Sigma 50935
По запросу
- Производитель: Sigma
- Каталожный номер:
50935 - CAS номер : 50-01-1
Краткое описание
Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride... Читать далее →
Характеристики реактива
...
Application
Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.
Other Notes
In the extraction and fractionation by cesium sulfate density gradient centrifugation of bovine nasal cartilage proteoglycan1; Induces subunit dissociation and unfolding of bovine liver glutamate dehydrogenase2; Dissociation of apolipoproteins of insect lipophorin3; Denaturation curve4.
Характеристики реактива | |
assay | ≥99.5% (AT) |
Molecular Formula | NH2C(=NH)NH2 · HCl |
pH | 4.5-6.0 (25 °C, 6 M in H2O) |