β-Xylosidase, thermostable recombinant, expressed in E. coli, ≥90% (SDS-PAGE) Sigma X3504
По запросу
- Производитель: Sigma
- Каталожный номер:
X3504 - CAS номер : 9025-53-0
Packaging
2 mg in glass bottle
Unit Definition
One xylosidase unit will produce 1 μmole of o-nitrophenol per minute at 70 °C from
a 1mM solution of o-nitrophenyl-β-xyloside in 50 mM sodium acetate at pH 5.8.
Physical form
Supplied as a solution in 50 mM Tris-HCl, pH 8.0, 100 mM NaCl, and 25% glycerol.
Biochem/physiol Actions
Releases reducing sugars from birchwood xylan ( X0502), also catalyzes the hydrolysis of 4-methylumbelliferyl-β-
β-Xylosidase undergoes post-translation glycosylation which has been shown to be critical for its proper activity and stability. Deglycosylation altered the the optimum temperature and pH for activity and decreased its thermostability. 1
Характеристики реактива | |
assay | ≥20 mg protein/mL (UV), ≥90% (SDS-PAGE) |
storage temp. | 2-8°C |