β-N-Acetylglucosaminidase from Canavalia ensiformis (Jack bean) ammonium sulfate suspension, ≥15 units/mg protein Sigma A2264
По запросу
- Производитель: Sigma
- Каталожный номер:
A2264 - CAS номер : 9012-33-3
Analysis Note
At pH 4.0, p-nitrophenyl β-N-acetylgalactosaminide is hydrolyzed at approximately 50% of the rate of hydrolysis of p-nitrophenyl β-N-acetylglucosaminide at pH 5.0.
Biochem/physiol Actions
This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates.
This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates. The activity of β-N-actylglucosaminidase may be determined with the chromogenic substrate p-nitrophenyl-N-acetyl-β-
Unit Definition
One unit will hydrolyze 1.0 μmole of p-nitrophenyl N-acetyl-β-
Physical form
Suspension in 2.5 M (NH4)2SO4, pH 7.0
Application
β -N-acetylglucosaminidase is a lysosomal enzyme used to hydrolyze N-acetyl-β-D-glucosaminides and N-acetyl-β-Dgalactosaminides. It is used in chemoenzymatic synthesis of oligosaccharides based on their effective transglycosylation of β-GlcNAc and β-GalNAcc. It may be a useful tool to study Alzheimer’s Disease 1. Acetylglucosaminidase from Canavalia ensiformis has been used to study enzymic detachment of biofilms 2.
Характеристики реактива | |
storage temp. | 2-8°C |