β-Lactamase from Enterobacter cloacae Type III, lyophilized powder, 6-18 units/mg protein (using benzylpenicillin) Sigma P4399
По запросу
- Производитель: Sigma
- Каталожный номер:
P4399 - CAS номер : 9073-60-3
Analysis Note
Protein determined by biuret
Application
β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression1. Product P4399 is produced from Enterobacter cloacae.
Biochem/physiol Actions
β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring1.
Packaging
5, 25 units in poly bottle
Preparation Note
Chromatographically purified
Unit Definition
One unit will hydrolyze 1.0 μmole of benzylpenicillin per min at pH 7.0 at 25 °C. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.
Physical form
Lyophilized powder containing sodium phosphate and sodium citrate buffer salts
Характеристики реактива | |
storage temp. | 2-8°C |