β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei lyophilized powder, ≥200 units/mg protein Sigma H9408
По запросу
- Производитель: Sigma
- Каталожный номер:
H9408 - CAS номер : 9028-38-0
Application
Suitable for the determination of acetoacetate and
Biochem/physiol Actions
In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Pseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).
Unit Definition
One unit will oxidize 1.0 μmole of
Physical form
lyophilized powder containing sucrose, β-NAD and Tris buffer salts
Характеристики реактива | |
storage temp. | −20°C |