β-Glucuronidase from Helix pomatia Type H-5, lyophilized powder, ≥400,000 units/g solid Sigma G1512
По запросу
- Производитель: Sigma
- Каталожный номер:
G1512 - CAS номер : 9001-45-0
Linkage
A further purification of G0876 by gel permeation chromatography.
Packaging
100000, 500000 units in poly bottle
Quality
Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Some preparations have been reported to contain acid phosphatase activity.
Unit Definition
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.
Application
β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.1
Biochem/physiol Actions
β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.2,3,4
β-Glucuronidase from Helix pomatia is a mixture of enzymes derived from the Roman snail.
Optimal pH:
• Glucuronidase activity: 4.5 to 5.0
• Sulfatase activity: ~6.2
Inhibitors:
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Характеристики реактива | |
storage temp. | −20°C |