β-Glucuronidase from Helix pomatia Type H-1, partially purified powder, ≥300,000 units/g solid Sigma G0751
По запросу
- Производитель: Sigma
- Каталожный номер:
G0751 - CAS номер : 9001-45-0
Packaging
100000, 500000, 1000000, 2000000 units in poly bottle
Quality
Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Some preparations have been reported to contain acid phosphatase activity.
Unit Definition
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.
Application
β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.1
Biochem/physiol Actions
β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.2,3,4
β-Glucuronidase from Helix pomatia is a mixture of enzymes derived from the Roman snail.
Optimal pH:
• Glucuronidase activity: 4.5 to 5.0
• Sulfatase activity: ~6.2
Inhibitors:
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
General description
β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Характеристики реактива | |
storage temp. | −20°C |