β-Glucuronidase from Escherichia coli ≥20,000 units/mg protein, recombinant, expressed in E. coli overproducing strain, lyophilized powder Sigma G8420
По запросу
Наличие: Срок поставки 60 дней
- Производитель: Sigma
- Каталожный номер:
G8420 - CAS номер : 9001-45-0
Packaging
25000, 100000 units in glass bottle
Quality
Contains buffer salts and stabilizer.
Unit Definition
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).
Application
β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.
Biochem/physiol Actions
β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.
Характеристики реактива | |
storage temp. | −20°C |