β-Galactosidase-biotin labeled from Escherichia coli lyophilized powder, 350-1200 units/mg protein Sigma G5025
Packaging
Package size based on protein content
Preparation Note
Biotinylated using biotin–maleimide (B 1267) which incorporates an extended spacer.
Unit Definition
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-
Physical form
Lyophilized powder containing Tris-acetate, DTT, MgCl2, and isopropyl β-
Application
β-Galactosidase was used as a control antigen in the selection of human antibody fragments by phage display.1
Biochem/physiol Actions
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.2
Характеристики реактива | |
storage temp. | −20°C |