β-Galactosidase from Escherichia coli for enzyme immunoassay, lyophilized, powder, ~140 U/mg Sigma 48275
По запросу
- Производитель: Sigma
- Каталожный номер:
48275 - CAS номер : 9031-11-2
Application
Aqueous solution may be used directly for derivatization without previous dialysis or gel permeation chromatography.
Other Notes
Used in a highly sensitive enzyme-linked immunoassay of human IgG with 4-methylumbelliferyl-β-D-
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Unit Definition
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 2-nitrophenyl-β-D-galacto
Biochem/physiol Actions
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.1
Physical properties
Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)
Характеристики реактива | |
grade | for enzyme immunoassay |
storage temp. | −20°C |