β-Galactosidase from Escherichia coli aqueous glycerol suspension, 600-1,200 units/mg protein (biuret) Sigma G4155
По запросу
- Производитель: Sigma
- Каталожный номер:
G4155 - CAS номер : 9031-11-2
Other Notes
Loss of enzymatic activity can occur if this enzyme is frozen. This enzyme is provided in a 50% glycerol solution and will not freeze if stored at -20° Celsius. It is not recommended that this enzyme preparation be stored in an ultracold freezer (-60 to -100° Celsius).
Packaging
5000, 15000 units in glass bottle
Unit Definition
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-
Physical form
Suspension in 50% glycerol, 5 mM Tris buffer salts, 5 mM magnesium chloride, 0.5 mM DTT, 0.5 mM mercaptoethanol. pH 7.4
Application
β-Galactosidase is used in the enzymatic assays in the synthesis of imidazolo-pyrrolidinoses.
Biochem/physiol Actions
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.1
Physical properties
Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)
Характеристики реактива | |
storage temp. | −20°C |