Aprotinin from bovine lung saline solution, 3-7 TIU/mg protein Sigma A6279
По запросу
- Производитель: Sigma
- Каталожный номер:
A6279 - CAS номер : 9087-70-1
Application
Aprotinin is largely used as an inhibitor of trypsin.
Caution
Aprotinin products should be stored at 2-8°C.
Preparation Note
This product is an aseptically filled solution in 0.9% NaCl and 0.9% benzyl alcohol. It has an activity of 3-7 TIU/mg protein and 5-10 TIU/mL solution.
Unit Definition
One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of Nalpha-benzoyl-DL-arginin
Biochem/physiol Actions
Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.
Характеристики реактива | |
Molecular Formula | C284H432N84O79S7 |
storage temp. | 2-8°C |