Aprotinin from bovine lung BioUltra, ≥4 TIU/mg solid, ≥98% (SDS-PAGE) Sigma A6106
По запросу
- Производитель: Sigma
- Каталожный номер:
A6106 - CAS номер : 9087-70-1
Application
Aprotinin is largely used as an inhibitor of trypsin.
Packaging
1 mg in glass bottle
25, 100 mg in poly bottle
Preparation Note
A further purification of A1153 to yield a product with slightly higher activity and purity
Unit Definition
One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of Nalpha-benzoyl-DL-arginin
One trypsin inhibitor unit (TIU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmole of N-α-benzoyl-
Biochem/physiol Actions
Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.
Характеристики реактива | |
assay | ≥98% (SDS-PAGE) |
Molecular Formula | C284H432N84O79S7 |
storage temp. | 2-8°C |