Amphotericin B from Streptomyces sp. ~80% (HPLC), powder Sigma A4888
По запросу
- Производитель: Sigma
- Каталожный номер:
A4888 - CAS номер : 1397-89-3
Biochem/physiol Actions
Amphotericin B binds to sterols, forming pores in the membrane, and causing small molecules to leak out. The product is most effective against fungi and yeast.
Caution
Amphotericin B remains active for 3 days in culture at 37°C. At -20°C, and with protection from air and light, the product remains active for 5 years.
Packaging
1, 5, 100 g in glass bottle
100, 250, 500 mg in glass bottle
Preparation Note
Amphotericin B is insoluble in water at pH 6 to 7, but soluble in water at pH 2 or 11. It is soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL). Aqueous solutions cannot be sterile filtered due to poor solubility.
Application
Amphotericin B is used to study the affects of the influx of calcium on antifungal activity, 1 and to study the binding and kinetics of Amphotericin B to sterols in monolayers, aqueous solutions, and phospholipid bilayers 2,3. It is also commonly used in tissue culture as an antifungal supplement.
Protocols & Applications
Характеристики реактива | |
assay | ~80% (HPLC) |
Molecular Formula | C47H73NO17 |
storage temp. | 2-8°C |