α-Lactalbumin from bovine milk For use as a marker in SDS-PAGE Sigma L6385
По запросу
- Производитель: Sigma
- Каталожный номер:
L6385 - CAS номер : 9051-29-0
Application
α-Lactalbumin from bovine milk is suitable for use:
• as an electrophoresis marker, with a molar mass of approximately 14,200Da1,2,3
• in a study to investigate selective binding of proteins on charged surface iron oxide nanoparticles via reverse charge parity model4
α-Lactalbumin was used in the reduction of the antigenicity of whey proteins by lactic acid fermentation.5
Biochem/physiol Actions
α-Lactalbumin consists of a single polypeptide chain with 8 cysteines which form disulfide bridges. α-Lactalbumin binds several metal ions, including calcium, which is thought to play a role in the regeneration of native α-lactalbumin from the reduced, denatured form.6 α-Lactalbumin also has a distinct zinc binding site that is thought to play a role in the binding of the lactose synthase complex. 7,8, The mature protein consists of 123 amino acid residues (14 kD), and it has a three-dimensional structure with 1.7 Angstrom resolution, demonstrating four a-helices and a triple stranded antiparallel β-sheet.9
Alters the substrate specificity of galactosyltransferase to increase the rate of lactose formation; the complex of galactosyltransferase and α-lactalbumin is called lactose synthase. Site-directed mutagenesis of Asp87 or Asp88 to Ala completely abolishes the strong calcium binding affinity and reduces the stimulation of lactose synthase to <3.5% of the maximal rate.
General description
α-Lactalbumin is a small, globular, whey protein that has been found in all milk studied to date. It is a metalloprotein of approximately 14 kDa produced in the mammary glands.9
Характеристики реактива | |
storage temp. | 2-8°C |